Senescent phenotype of astrocytes leads to microglia activation and neuronal death

Wenyou Zhang; Xuehan Yang; Jingyue Liu; Yichen Pan; Ming Zhang; Li Chen

doi:doi:10.18632/aging.

← Back

Research Paper

Senescent phenotype of astrocytes leads to microglia activation and neuronal death

Figure 5. The direct effect of aged astrocytes in communicating with neurons. (A) A schematic diagram of the interaction of cell supernatants. (B) Cell viability assay of Neuro-2a cells by using astrocytes treated with or without H₂O₂ supernatant. (C) Caspase-3 activity in Neuro-2a cells (treated with astrocytes-CM for 24 h). Green fluorescence indicates the activity of caspase-3. Blue indicates nuclear stained by Hoechst33342. Merge is the overlapped green and blue. ×200 magnification. All experiments were expressed as the mean +/−S.D, analyzed by ANOVA followed by Tukey’s test, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns represents no significance.