Research Paper Volume 14, Issue 18 pp 7378—7389
Fasudil inhibits hepatic artery spasm by repressing the YAP/ERK/ ETA/ETB signaling pathway via inhibiting ROCK activation
Figure 3. Fasudil repressed the YAP1 signaling pathway in arterial smooth muscle tissues of rabbits. (A) GSE60887 dataset downloaded from the GEO database. The microarray analysis revealed that MAPK1 expression declined in Fasudil group compared with that in Sham group. P<0.05: Fasudil group vs. Sham group. (B, C) Decreased MYC and YAP1 expressions in Fasudil group in comparison with those in Sham group. P<0.05: Fasudil group vs. Sham group. (D, E) Co-expression analysis revealed that MAPK1 was correlated with YAP1 and MYC. (F) Immunofluorescence staining for YAP1 andα-SMA. Compared with that in Model group and Sham group, the YAP1-positive fluorescence area was significantly decreased after Fasudil treatment. P<0.05: Fasudil group vs. Sham group. (G) Western blotting demonstrated that YAP1 protein expression level was decreased in Fasudil group in contrast with that in Model group. P<0.05: Fasudil group vs. Model group.