Research Paper Volume 14, Issue 15 pp 6149—6168
Interleukin-17D promotes lung cancer progression by inducing tumor-associated macrophage infiltration via the p38 MAPK signaling pathway
Figure 5. IL-17D activates p38 MAPK signaling pathway in lung cancer. (A) Volcano plot assessment of mRNA expression in A549 cells between IL-17D overexpression and control groups. (B) Significantly enriched KEGG pathways relative to differentially expressed mRNAs upon IL-17D overexpression. Y-axis represents pathways; X-axis represents the amount of the mRNAs enriched in KEGG pathways. (C) Immunoblots showing the expression of p38, p-p38, Flag and β-actin. Left, A549 cells were transduced with IL-17D or empty vector. Right, LLC1 cells were transduced with IL-17D or empty vector. (D) Immunoblots showing the expression of p38, p-p38, IL-17D and β-actin. H1155 cells were transduced with control or shRNAs against IL-17D. (E) Immunostaining of IL-17D and p-p38 in human lung cancer tissues. Scale bars are 20 μm. (F) Semiquantitative analysis of immunostaining revealed that IL-17D scores were associated with p-p38 scores (r = 0.5998, P < 0.0001).