Research Paper Volume 14, Issue 15 pp 6006—6027

Figure 5. ABT263 and D+Q decreased pain-related activation of DRG neurons in age-associated OA mice. (A) Representative immunofluorescence images for CGRP, NGF, and TrkA of L4 DRG in 21 to 22-month-old mice administered by ABT263 or Veh. White dots indicate a DRG region. (B–D) Quantitative analysis of CGRP+ (n = 4 mice for Veh, n = 6 mice for ABT263), NGF+ (n = 4 for Veh, n = 5 for ABT263), and TrkA+ neurons (n = 4 for Veh, n = 6 for ABT263) in L4 DRG of ABT263 or Veh-treated aged mice. (E) Immunofluorescence analysis of CGRP +, NGF+, and TrkA+ neurons (red) in L4 DRG of D+Q or Veh-treated aged mice. (F–H) Quantification of CGRP+, NGF+, and TrkA+ neurons in L4 DRG of D+Q or Veh-treated aged mice (n = 4 for Veh, n = 6 for D+Q). Whisker plots represent the 10^th and 90^th percentiles, and the line corresponds to the median. Unpaired Student t-test was used for statistical analysis. * p < 0.05, ** p < 0.01; Unpaired Student’s t-test. Scale bars, 100 μm.