Research Paper Volume 14, Issue 18 pp 7364—7377

Figure 4. Effect of M1-Exo-GEM on MB49 apoptosis. (A) In PI staining, M1-Exo and GEM could promote apoptosis, as manifested by significantly higher number of positive cells than that of Control group, while M1-Exo-GEM could further promote apoptosis. (B, C) According to protein assay results, Bcl-2 was highly expressed in the Control group, while Caspase-3 and Bax were lowly expressed. GEM and M1-Exo enhanced the expressions of Caspase-3 and Bax and inhibited the Bcl-2 expression, suggesting their pro-apoptosis effect. M1-Exo-GEM could further promote the apoptosis signal activation. *P<0.05 vs. Control group.