Research Paper Volume 14, Issue 13 pp 5523—5536

Figure 6. Silence of CHST7 resensitized OVA-resistant gastric cancer cells to oxaliplatin. (A, B) The knockdown efficiency of CHST7 in AGS/OXA and MKN-45/OXA cells transfected with CHST7 siRNAs (si-CHST7-1 and si-CHST7-2) or si-NC was validated by qRT-PCR and western blot. (C–F) Cell viability and the IC₅₀ of oxaliplatin in AGS/OXA and MKN-45/OXA cells transfected with CHST7 siRNAs or si-NC were determined by CCK-8 assay. (G) Colony formation assay showed knockdown of CHST7 decreased cell survival of AGS/OXA and MKN-45/OXA cells exposed to 15μM oxaliplatin. (H) Caspase-3 activity assay indicated that knockdown of CHST7 increased apoptosis of AGS/OXA and MKN-45/OXA cells exposed to 15μM oxaliplatin. (I, J) qRT-PCR indicated that silence of CHST7 decreased expression levels of drug-resistant genes (MDR1, MRP1, LRP1) in AGS/OXA and MKN-45/OXA cells. Two-way ANOVA for (C, E) Student’s t-test for others.