Research Paper Volume 14, Issue 13 pp 5511—5522
Detachment stress mediated bioenergetic switch of malignant melanoma cells into anti-Warburg phenotype
Figure 3. Detachment stress decreased glycolytic capacity but increased respiratory capacity of melanoma A375 cells investigated by Seahorse XF24 analyzer. (A) ECAR analysis showed higher rate of glycolysis in adherent melanoma cells. (B) OCR analysis showed higher oxygen consumption in suspended melanoma cells. (C) Decreased glycolytic capacity was observed in suspended melanoma cells, but ~80% glycolytic capacities were used in both adherent and suspended melanoma cells. (D) Increased respiratory capacity was seen in suspended melanoma cells. Adherent melanoma cells used full capacity in oxygen consumption, while suspended melanoma cells used only 46.7% of capacity. (E) Metabolism in suspended melanoma cells was highly dependent on mitochondrial respiration. Data were mean ±S.D. (n=3).