Research Paper Volume 14, Issue 13 pp 5511—5522
Detachment stress mediated bioenergetic switch of malignant melanoma cells into anti-Warburg phenotype
Figure 2. Decreased lactate release and gene expression in suspended melanoma A375 cells. (A) Reduced lactate secretion from suspended melanoma cells was seen under neutral pH condition (pH7.0). while at pH8.5, the maximum levels of lactate secretions were achieved in both adherent and suspended melanoma cells. Data were mean ±S.D. (n=3); **, p < 0.01. Gene expression was examined by (B) qPCR and (C) western blot. Data were mean ±S.D. (n=3); **, p < 0.01.