Research Paper Volume 14, Issue 13 pp 5493—5510
E3 ubiquitin ligase RBX1 drives the metastasis of triple negative breast cancer through a FBXO45-TWIST1-dependent degradation mechanism
Figure 3. RBX1 exerts its oncogenic role by enhancing EMT in TNBC. (A, B) Western blot assay of the phenotypic markers of stable RBX1 silencing, containing N-cadherin and E-cadherin, in BT549 and MDA-MB-231 cells. Expression of tubulin was applied as a loading control. (C, D, F) Confocal microscopy assay of vimentin and E-cadherin in TNBC cells with the overexpression or knockdown of RBX1. Green and red signals reveal staining for the associated proteins, blue signal denotes the nuclear DNA staining for DAPI. (E) Western blot assay of the phenotypic markers, containing N-cadherin and E-cadherin in SUM159PT cells transfected with plasmid with the overexpression of RBX1. (G) Western blot assay of the phenotypic markers, containing N-cadherin and E-cadherin.