Research Paper Volume 14, Issue 13 pp 5493—5510

Figure 2. RBX1 facilitated the cell invasion and migration of TNBC in vivo and in vitro. (A, B) RBX1 mRNA and protein levels in normal breast epithelial, TNBC, and non-TNBC cell lines. ^***P<0.001. (C, D) The analysis of qRT-PCR and western blot were applied for determining the RBX1 expression levels of in BT549 and MDA-MB-231 cells stably transfected with the plasmid silenced by RBX1. ^*P < 0.05, ^**P < 0.01. (E, F) The assays of Transwell invasion and migration of BT549 and MDA-MB-231 cells transfected by the plasmid with knockdown of RBX1. ^*P < 0.05, ^**P < 0.01. (G, H) The assays of Transwell invasion and migration of SUM159PT cells transfected by plasmid with overexpression of RBX1. ^*P < 0.05, ^**P < 0.01. (I) H&E staining of sections embedded with paraffin of metastatic nodules in the lungs. ^*P < 0.05.