Figure 5. Hedgehog signaling is required for BDDD-721 against medulloblastoma cells. (A) The cell viability of DAOY and ONS-76 cells were analyzed by MTT assay in DAOY and ONS-76 cells treated with BDDD-721 and/or SAG. (B) The cell viability of DAOY and ONS-76 cells were analyzed by MTT assay in DAOY and ONS-76 cells treated with BDDD-721 and/or cyclopamine. Cell apoptosis was detected by flow cytometry in DAOY (C) and ONS-76 (D) cells treated with BDDD-721 and/or SAG. Then, cell apoptosis was detected by flow cytometry in DAOY (E) and ONS-76 (F) cells treated with BDDD-721 and/or cyclopamine. (G) Column chart represents the percentage of apoptosis cells analyzed by SPSS statistical software.