Figure 4.Effects of BDDD-721 on the hedgehog pathway in medulloblastoma cells. (A) Gli activity was detected by Gli-luciferase reporter assay in medulloblastoma cells treated with curcumin or BDDD-721. (B) The expression of Shh protein was analyzed by western blot in DAOY and ONS-76 cells treated with BDDD-721 for 48h. (C) Proteins of Smo, GLI1 and Ptch1 were examined by western blot in DAOY and ONS-76 cells treated with BDDD-721 for 48h. (D) Proteins of N-myc, Bcl-2 and Cyclin D1 were quantified by western blot in DAOY and ONS-76 cells treated with BDDD-721 for 48h.
