Research Paper Volume 14, Issue 13 pp 5464—5477
Curcumin analogue BDDD-721 exhibits more potent anticancer effects than curcumin on medulloblastoma by targeting Shh/Gli1 signaling pathway
Figure 2. BDDD-72 and curcumin triggered apoptosis against medulloblastoma cells. (A) Cell apoptosis was examined by flow cytometry assay using the Annexin V/PI after BDDD-721 or curcumin treatment for 24h. (B) Apoptosis-related proteins of cleaved PARP, PARP and Bcl-2 were analyzed by western blot. (C) Caspase-3 activity was measured by ELISA assay. Compared with Control, *p < 0.05, **p < 0.01.