Research Paper Volume 14, Issue 13 pp 5464—5477
Curcumin analogue BDDD-721 exhibits more potent anticancer effects than curcumin on medulloblastoma by targeting Shh/Gli1 signaling pathway
Figure 1. Effects of BDDD-721 and curcumin on medulloblastoma cells. (A) Chemical structures of BDDD-721. (B) Effects of BDDD-721 and curcumin on DAOY, UW402, UW473 and ONS-76 cells were analyzed by MTT assay in vitro. (C) Effects of BDDD-721 and curcumin on cell viability were evaluated by EdU assay. (D) Column chart represents the percentage of EdU positive cells analyzed by SPSS statistical software. (E) The proliferation antigen Ki67 and PCNA were detected by western blot. (F) Cell cycle was analyzed by flow cytometry following propidium iodide staining for DNA content after BDDD-721 treatment for 24h. (G) Cell cycle associated proteins of Cyclin D1, CDK4, and CDK6 were examined by western blot. Compared with Control, *p < 0.05, **p < 0.01.