Research Paper Volume 14, Issue 13 pp 5449—5463
Long non-coding RNA H19 contributes to spinal cord ischemia/reperfusion injury through increasing neuronal pyroptosis by miR-181a-5p/HMGB1 axis
Figure 8. Downregulation of H19 decreased inflammation response by upregulation of miR-181-5p in SCI/R mice. (A–C) The expression levels of H19, miR-181a-5p, and HMGB1 in the spinal cord tissues of mice following SCI/R were determined by RT-qPCR and western blot analysis. (D–F) The levels of IL-1β, IL-18, TNF-α in serum of mice were determined by ELISA assay. (G–J) The protein levels of NLRP3, ASC, and caspase-1 were determined by Western blotting. The results were presented as the mean ± SD. N = 10; *P<0.05.