Research Paper Volume 14, Issue 13 pp 5449—5463
Long non-coding RNA H19 contributes to spinal cord ischemia/reperfusion injury through increasing neuronal pyroptosis by miR-181a-5p/HMGB1 axis
Figure 4. MiR-181a-5p attenuates pyroptosis caused by OGD/R. PC12 cells were transfected with miR-181a-5p mimic or miR-181a-5p inhibitor or their negative control and then subjected to OGD/R. (A) Relative levels of miR-181a-5p in transfected PC12 cells following OGD/R exposure. (B–D) The levels of IL-1β, IL-18, TNF-α were determined in transfected PC12 cells following H/R exposure by ELISA. (E–H) The protein levels of NLRP3, ASC, and caspase-1 were determined in transfected PC12 cells by Western blotting. (I) Cell apoptosis rate was identified by TUNEL assay. The results were presented as the mean ± SD. N = 3; *P<0.05.