Research Paper Volume 14, Issue 13 pp 5449—5463
Long non-coding RNA H19 contributes to spinal cord ischemia/reperfusion injury through increasing neuronal pyroptosis by miR-181a-5p/HMGB1 axis
Figure 3. H19 acted as miR-181a-5p sponge to suppress its expression. (A) The sequences H19 containing the miR-181a-5p binding sites or mutant binding sites were showed. (B) The expression of miR-181a-5p was detected by RT-qPCR. (C) The correlations of the H19 and miR-181a-5p expression levels in the mouse spinal cord tissues following I/R were analyzed by Pearson correlation analysis. (D, E) luciferase reporter gene assay and RNA pull-down were used to detect the interaction between miR-181a-5p and H19 in PC12 cells. (F) The expression of miR-181a-5p was detected by RT-qPCR. The results were presented as the mean ± SD. N = 3; *P < 0.05.