Research Paper Volume 14, Issue 15 pp 5966—5983

Figure 2. Immunohistochemistry for markers of autophagy on mouse knee joint sections. Representative images of medial compartment of knee joints from BL/6^C57 and conplastic (BL/6^NZB) mice at 90 weeks of age stained with LC3 (A), Beclin-1 (B) and P62 (C). Quantitative analysis of LC3-positive cells (D), Beclin-1-positive cells (E), and P62-positive cells (F) of knee joints from BL/6^C57 and conplastic (BL/6^NZB) mice at 90 weeks. Original magnification: 20×. Scale bar, 50 μm. Black arrow indicates positively stained chondrocyte. Chondrocyte magnification (40×) is shown in the bottom-left corner of the images. Graphs represent means ± SEM; n=5 in BL/6^C57 and n=6 conplastic (BL/6^NZB) mice for LC3 and Beclin-1, and n=6 in BL/6^C57 and n=6 conplastic (BL/6^NZB) mice for P62. *p<0.05; **p<0.01 by non-parametric Mann-Whitney test.