Research Paper Volume 14, Issue 13 pp 5406—5416

Figure 5. circEPSTI1 facilitates cervical cancer progression and cisplatin resistance through circEPSTI1-miR-370-3p-MSH2 axis. (A, B) CCK-8 assay for assessment of rate of cell proliferation in SiHa, as well as HT-3 cervical cancer cell lines. (C, D) Inhibition of circEPSTI1 remarkably increased the responsivity of cervical cancer to cisplatin treatment, whilst this effect could be rescues by miR-370-3p inhibitors in SiHa along with HT-3 cell lines of cervical cancer. (E) Expression of MSH2 mRNA transcript was reduced after circEPSTI1 inhibition and increased following the transfection with inhibitors of miR-370-3p, detected by qPCR analysis. (F) Knockdown of circEPSTI1 downregulated the MSH2 protein expression, which could be reversed through the miR-370-3p inhibitors in SiHa cervical cancer cells, illustrated by the western blot analysis.