Research Paper Volume 14, Issue 13 pp 5406—5416

Figure 3. circEPSTI1 sponges miR-370-3p in cervical cancer. (A, B) U6, ACTB, circEPSTI1 and EPSTI1 mRNA contents in nuclear as well as cytoplasmic fractions were assessed via qRT-PCR assays in SiHa and HT-3 cell lines of cervical cancer. (C) Predicted docking sites of miR-370-3p within the circEPSTI1 sequence. (D) The contents of miR-370-3p in ten paired cervical cancer samples and vicinal non-malignant tissues. (E) The relative contents of miR-370-3p in non-malignant HcerEpic cell line and cervical cancer cell lines. (F, G) Dual luciferase reporting experiments revealed that the relative amount of fluorescence value was reduced after transfected with miR-370-3p in SiHa, as well as HT-3 cervical cancer cell lines. (H) MS2-based RNA immunoprecipitation assays were conducted to prove the docking of miR-370-3p on circEPSTI1 in SiHa along with HT-3 cell lines of cervical cancer.