Research Paper Volume 14, Issue 13 pp 5390—5405

Figure 6. Silencing MIR503HG activated MAPK and PI3K/AKT pathways by increasing TMEFF1 expression. SKOV3 and OVCAR3 cells were transfected with MIR503HG siRNA alone or together with TMEFF1 siRNA, after transfection for 48 h, (A, B) the expression of phosphorylated RAF (p-RAF), p-MEK, p-ERK1/2, p-PI3K, and p-AKT, and total RAF (p-RAF), t-MEK, t-ERK1/2, t-PI3K, and t-AKT was assessed with Western blotting. ^*P < 0.05. n = 6 in each group. Each test was repeated at least three times independently.