Research Paper Volume 14, Issue 13 pp 5390—5405

Figure 2. MIR503HG overexpression alleviated malignant behaviors of ovarian cancer cells. pcDNA-MIR503HG, MIR503HG siRNA and respective controls were transfected into SKOV3 and OVCAR3 cells, after transfection for 48 h, (A) MIR503HG expression was measured with RT-qPCR. (B) CCK-8 was performed to detect cell proliferation. (C) Cell invasion was determined with Transwell assay. (D) Flow cytometry was used to analyze cell apoptosis. (E) The protein levels of E-cadherin, N-cadherin and Vimentin were assessed with Western blotting. ^*P < 0.05, ^**P < 0.01. n = 6 in each group. Each test was repeated at least three times independently.