Research Paper Volume 14, Issue 11 pp 4914—4926

Figure 5. MiR-485-3p was the sponge target of circSOX9 in NPC cells. (A) Use predictive software (CircInteractome, Starbase) for bioinformatics analysis of potential target genes. (B) qRT-PCR analysis of target miRNAs in NPC tumors and para-tumor tissues. (C) Dual-luciferase reporter gene assay detects the interaction of circSOX9 and miR-485-3p, miR-577, and miR-582-3p. (D) qRT-PCR analysis of the expression correlation of circSOX9 and miR-485-3p in NPC. (E) The binding site between circSOX9-wt and miR-485-3p, and the mutant sequence (circSOX9-mut) that cannot bind to miR-485-3p was designed. (F) The dual-luciferase reporter gene assay proved the direct binding between circSOX9 and miR-485-3p in HONE1 and CNE2 cells. (G) RIP assay verified the combination of circsox9 and miR-485-3p with Ago2).^*P < 0.05, ^**P < 0.01, ^***P < 0.001.