Research Paper Volume 14, Issue 13 pp 5376—5389
An active ingredient isolated from Ganoderma lucidum promotes burn wound healing via TRPV1/SMAD signaling
Figure 7. Effect of TRPV1 inhibitor on HaCaT cell proliferation. (A) HaCaT cells were pre-incubated with TRPV1 inhibitor (0.0258 μg/mL) for 3 and 16 h, followed by further incubation with GLSO for 48 h. GLSO-promoted cell proliferation was downregulated by TRPV1 inhibitor. (B) Quantitative analysis of reduced HaCaT cell numbers treated with TRPV1 inhibitor. (C) Immunofluorescence staining for TRPV1 and SMAD2/3 expression treated with TRPV1 inhibitor. Treatment with GLSO increased TRPV1 and SMAD2/3 expression that were down regulated by TRPV1 inhibitor. Scale bar=200 μm. The data are presented as the mean ± SD. *P<0.05, **P<0.01 versus control.