Research Paper Volume 14, Issue 11 pp 4858—4873
A novel ALG10/TGF-β positive regulatory loop contributes to the stemness of colorectal cancer
Figure 7. ALG10 and TGF-β signaling forms a positive ALG10/TGF-β regulatory loop by being bound by Smad2. (A and B) ALG10 mRNA and protein levels were examined in CRC cells with the treatment of different concentrations of TGF-β. (C) The diagram of Smad2 binding sites on ALG10 gene promoter. (D) Luciferase reporter assay was constructed to detect regulation of Smad2 on the regulation of different truncations of ALG10 gene promoter as indicated. (E) ChIP analysis were performed to determine the binding of Smad2 on ALG10 gene promoter. (F) Sphere size was measured in CRC cells with TGF-β treatment plus ALG10 knockdown or not. (G) CD133+ positive cells were evaluated in the cells described in (F). n = 3, **P < 0.01 vs. Control.