Research Paper Volume 14, Issue 11 pp 4858—4873

Figure 4. ALG10 knockdown attenuated the chemoresistance of CRC cells in vitro. (A and B) ALG10 mRNA and protein levels were examined in HCT-116 and HCT-116-OR cells. (C and D) The mRNA and protein levels of stemness markers were detected in HCT-116 and HCT-116-OR cells. (E and F) Sphere size and number were determined in HCT-116 and HCT-116-OR cells. (G) CD133+ subpopulation was evaluated in HCT-116 and HCT-116-OR cells. (H) ALDH activity was measured in HCT-116 and HCT-116-OR cells. (I) The IC₅₀ value of oxaliplatin was calculated in HCT-116-OR cells. (J) The IC₅₀ value of oxaliplatin was measured in HCT-116-OR-ALG-kd cells. n = 3, ^**P < 0.01 vs. Control.