Research Paper Volume 14, Issue 11 pp 4858—4873
A novel ALG10/TGF-β positive regulatory loop contributes to the stemness of colorectal cancer
Figure 2. ALG10 knockdown blocks the stemness of CRC cells. (A) The knockdown efficiency of ALG10-kd was confirmed in CRC cells through western blot assay. (B) The protein expression of stemness markers (Oct4, Sox2, Nanog) was detected in CRC cells with or without ALG10 knockdown. (C and D) The mRNA expression of stemness markers (Oct4, Sox2, Nanog) was measured in CRC cells with or without ALG10 knockdown. (E) ALDH activity was determined in CRC cells with or without ALG10 knockdown. (F and G) Sphere formation ability was evaluated in CRC cells with or without ALG10 knockdown via measuring sphere size and counting sphere number. (H) CD133+ cell sub-population was analyzed in CRC cells with or without ALG10 knockdown. n = 3, **P < 0.01 vs. Control.