Research Paper Volume 14, Issue 11 pp 4755—4768
LINC00094/miR-19a-3p/CYP19A1 axis affects the sensitivity of ER positive breast cancer cells to Letrozole through EMT pathway
Figure 5. Overexpression of LINC00094 affects IC50 of breast cancer cells to Letrozole. (A) The cell healing rate at 0 hours and 72 hours was measured by scratch assay with the scale of 100 μm. The experiment was repeated 3 times. **P<0.01, ****P<0.0001. (B) Transwell assay was performed to detect the effect of LINC00094 overexpression on the mobility of breast cancer cells, 20× microscope field, and the experiment was repeated 3 times. ****P<0.0001. (C) Cell clonal formation assay was performed to detect the effect of overexpression of LINC00094 on the colony formation of breast cancer cells. The experiment was repeated for 3 times.****P<0.0001. (D) CCK-8 was used to detect cell activity, and each group had 3 repeated wells.**P<0.01. (E) Graphpad Prism calculated IC50 of MCF-7 and BT-474 cells against Letrozole. ****P<0.0001.