Research Paper Volume 14, Issue 11 pp 4755—4768
LINC00094/miR-19a-3p/CYP19A1 axis affects the sensitivity of ER positive breast cancer cells to Letrozole through EMT pathway
Figure 4. Adsorption of miR-19a-3p by LINC00094 sponge in breast cancer. (A) Bioinformatics analysis of LINCRNA binding to miR-19a-3p. (B) RT-QPCR was used to detect the expression levels of miR-19a-3p (U6 as internal reference) and LINC00094 (β-actin as internal reference) in plasma of breast cancer patients. (C) Endogenous expression of LINC00094 in breast cancer cells was detected by RT-QPCR. *P<0.05, **P<0.01, ***P<0.001. (D) miRNA specific PCR detection of miR-19a-3p expression level (U6 as internal reference). **P<0.01, ****P<0.0001. (E) Binding sequence of miR-19a-3p and LINC00094, and mutation site of mutant LINC00094. (F) Luciferase activity test of HEK-293T cells in each transfection group, ****P<0.0001.