Emergency SARS-CoV-2 variants of concern: rapidly direct RT-qPCR detection without RNA extraction, clinical comparison, cost-effective, and high-throughput

Bing-Heng Yang; Hsing-Yi Chung; Li-Ting Kao; Ming-Jr Jian; Chih-Kai Chang; Jung-Chung Lin; Kuo-Ming Yeh; Chien-Wen Chen; Ya-Sung Yang; Shan-Shan Hsieh; Sheng-Hui Tang; Cherng-Lih Perng; Feng-Yee Chang; Hung-Sheng Shang

doi:doi:10.18632/aging.204095

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COVID-19 Research Paper Volume 14, Issue 11 pp 4624—4633

Emergency SARS-CoV-2 variants of concern: rapidly direct RT-qPCR detection without RNA extraction, clinical comparison, cost-effective, and high-throughput

Figure 1. Detection of SARS-CoV-2 using two methods on two different machines. Data are depicted as scattered dot plots with stated mean values. Each dot represents one C_t value corresponding with one specimen and p-values were found using paired t-test. (A) Shows SARS-CoV-2 N1 gene results. (B) Shows SARS-CoV-2 E gene results.