Research Paper Volume 14, Issue 9 pp 4137—4157

Figure 4. Overexpression of CRART16 in gastric cancer cells promotes angiogenesis. (A) 3D spheroid sprouting assay showed that conditioned medium from CRART16-overexpressing gastric cancer cells induced more prominent spheroid sprouting of HUVECs, and vice versa (^*P < 0.05). (B) Endothelial cell tube formation assay showed that conditioned medium from CRART16-overexpressing gastric cancer cells induced a more significant tube-like structures formation of HUVECs. Conversely, CRART16-silencing had the opposite effects on tube-like formation (^*P < 0.05). (C) The invasive capability of HUVECs was determined using the transwell invasion assay (^*P < 0.05). (D) Chick chorioallantoic membrane (CAM) assays. Newly formed vessels per field were quantified (^*P < 0.05). All data are presented as mean ± SD from three separate experiments.