Research Paper Volume 14, Issue 9 pp 3801—3812

Figure 1. OPE inhibits the proliferation and induces S phase arrest in A20 cells. (A–C) OPE inhibits the proliferation of A20 cells. A20 cells were treated with different concentrations of OPE (0, 6.25, 12.5, 25, 50, and 100 μg/mL) for 24 h (A), 48 h (B), and 72 h (C), respectively, and the cell viability was examined by CCK-8 assay. (D, E) OPE induces S-phase arrest in A20 cells. A20 cells were treated with different concentrations of OPE (0, 25, 50, and 100 μg/mL) for 48 h, and cell cycle distribution was accessed by flow cytometry. (F) Western blot was carried out to detect the expression of cell cycle-associated proteins. Data are presented as means ± SD of at least three independent experiments. (^*p < 0.05; ^**p < 0.01; ^***p < 0.001, compared to the untreated control).