Research Paper Volume 14, Issue 7 pp 3259—3275

Figure 1. LAP3 was upregulated in 6-month HFD-induced E3 rats with NASH. (A) H&E staining of liver specimen for each animal group. (B) The score for hepatic steatosis, hepatocytes ballooning and lobular inflammation were analyzed (n = 10 per group). (C and D) The protein expression of LAP3 in livers of 6-month HFD induced NASH E3 rats were evaluated by Western blotting (C) and normalized by β-actin (D) (n = 11 for each group). (E) Increased LAP3 is predominantly expressed in hepatocyte nucleus in 6-month HFD induced NASH E3 rats through Immunohistochemistry (n = 10 per group). (F) Statistics for immunohistochemistry result by Image pro plus. (G) lap3 mRNA expression in livers of 6-month HFD induced NASH E3 rats was detected by RT-qPCR (n = 10 per group). (H and I) Serum LAP3 content in 6-month HFD induced NASH E3 rats was detected by western blotting (F) and normalized by serum total protein (G) (n = 10 per group). Data are expressed as means ± SD from three independent experiments. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001. Abbreviations: HFD-NASH: high-fat-diet-induced nonalcoholic steatohepatitis; RT-qPCR: quantitative Realtime PCR.