Research Paper Volume 14, Issue 7 pp 3216—3232

Figure 6. Trehalose inhibits the ferroptosis of neuronal cells induced by Erastin. (A) CCK8 method is used to assess cell viability; (B) PI staining of neuronal cells; (C) Quantification of PI positive cells; (D, E) Flow cytometry to detect ROS level and quantitative analysis; (F–H) GSH, MDA and Fe²⁺ measurement; (I–K) Western blot to detect the expression of Acsl4 and Gpx4 protein and quantitative protein analysis; (L, M) PCR to detect Acsl4 mRNA and Gpx4 mRNA expression. ^*p < 0.05 compared with the Control group, ^#p < 0.05 compared with the Erastin group.