Research Paper Volume 14, Issue 7 pp 3143—3154

Figure 5. lncMIAT/miR-216a axis regulates p38MAPK expression in PBMCs. (A) Targetscan 7.2 predicted a binding site of 3’UTR of p38MAPK by miR-216a. And mutant 3’UTR of p38MAPK was prepared for further analysis. (B) Luciferase assays were performed with wild type or mutant 3’-UTR of p38MAPK, to examine the impact of miR-216a for luciferase activity. (C) Cells were transfected with wild-type or mutant miR-216a and 3’-UTR of p38MAPK. RNA pull-down assays were performed for the interacted 3’-UTR of p38MAPK. (D) RT-qPCR assays were performed for p38MAPK expression levels in miR-216a or inhibitor transfected cells. (E, F) lncMIAT and miR-216a mimics were co-transfected in PBMCs as indicated. The expression levels of p38MAPK mRNA were detected by RT-qPCR (E) and Western blot (F). *, p < 0.05.