Research Paper Volume 14, Issue 7 pp 3143—3154

Figure 3. lncMIAT increases p38MAPK expression to upregulate inflammatory cytokine secretion. (A) The expression levels of AMPK, p38MAPK and corresponding phosphorylated proteins were measured in the lncMIAT overexpressing and silencing PBMCs with Western blot assays. GAPDH was used as loading control. (B) The mRNA expression of p38MAPK was analyzed with the transfected PBMCs and corresponding control cells with PT-qPCR assays. (C) CCK-8 assays were performed to evaluate the PBMCs cell viability, which were treated with Doxorubicin (1 μM) for 24h or 48h. (D) p38MAPK knockdown PBMCs were established. The p38MAPK protein levels were confirmed with Western blot assays. GAPDH was used as control. (E, F) The inflammatory cytokine secretion of IL-6, TNF-α and IL-1β were measured with ELISA assays in the transfected PBMCs, which were treated with Doxorubicin (1 μM). *, p < 0.05.