Research Paper Volume 14, Issue 4 pp 1812—1821
Qi Ling decreases paclitaxel resistance in the human prostate cancer by reversing tumor-associated macrophages function
Figure 4. IL-6 supplement reverted Qi Ling serum-induced phenotype transition of TAMs in tumor milieu. In the same co-culture system above: TAMs were treated with PBS+vehicle, Qi Ling+vehicle or Qi Ling+IL-6. (A and B) mRNA levels of M1 markers (iNOS and TNFα) and M2 markers (IL-10 and CCL22) in TAMs of each group were determined by qRT-PCR. (C and D) Protein levels of M1 markers (iNOS and TNFα) and M2 markers (IL-10 and CCL22) in TAMs of each group were measured by ELISA. The data represent the mean ± SD. **p < 0.01 determined by Student’s t-test.