Research Paper Volume 14, Issue 1 pp 509—525

Figure 6. LAMC1 is directly restrained by miR-376a-3p. (A) Knockdown efficiency and amplification efficiency of LAMC1 in CCA cells both at mRNA and (B) protein level. (C) miR-376a-3p regulated LAMC1 expression both at mRNA and (D) protein level. (E) LAMC1 expression in TCGA database. (F) The expression of LAMC1 in CCA tissues both at mRNA and (G) protein level. (H) LAMC1 expression was negatively correlated with miR-376a-3p expression. (I) The expression of LAMC1 in CCA cells both at mRNA and (J) protein level. (K) The wild type and mutant type of LAMC1 3’UTR were cloned into luciferase reporter plasmids, respectively. (L) The miR-376a-3p mimics reduced the luciferase activity of LAMC1 wild type rather than mutant type. (M) RIP assay uncovered that LAMC1 was enriched by anti-AGO2 antibodies with miR-376a-3p mimics in CCA cells. ^**P < 0.01, ^***P < 0.001.