Research Paper Volume 14, Issue 1 pp 509—525

Figure 4. PSMA3-AS1 competitively binds to miR-376a-3p in CCA cells. (A) Subcellular fractionation assay showed that PSMA3-AS1 was mainly expressed in the cytoplasm of CCA cells. (B) PSMA3-AS1 dramatically inhibited miR-376a-3p expression in CCA cells. (C) The expression of miR-376a-3p in CCA tissues. (D) miR-376a-3p expression was negatively correlated with PSMA3-AS1 expression. (E) The expression of miR-376a-3p in CCA cells. (F) Knockdown efficiency and amplification efficiency of miR-376a-3p in CCA cells. (G, H) The luciferase reporter assay indicated that the luciferase activity of PSMA3-AS1 wild type was repressed by co-transfection with miR-376a-3p mimics, but the luciferase activity of PSMA3-AS1 mutant type was not affected. (I) AGO2 RIP assay further demonstrated the direct interaction between PSMA3-AS1 and miR-376a-3p. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.