Research Paper Volume 14, Issue 1 pp 497—508

Figure 5. PPP6C could be downstream of miR-208a-3p and involves in CRC tumor progression. (A) CCK-8 assay was performed to detect the cell viability in CRC cells. n = 6. ^*P < 0.05, ^**P < 0.01. (B) Cell cycle was determined in CRC cells by flow cytometry. n = 5. ^*P < 0.05 vs. si-NC group, ^#P < 0.05 vs. si-miR group. n.s. indicated no significance. (C) The clone formation assay was used to assess the cell proliferation. (D) The EdU assay results in CRC cells. n = 5. ^*P < 0.05 vs. si-NC group, ^#P < 0.05 vs. si-miR group (E) The flow cytometry was performed to detect the apoptosis level in CRC cells. n = 5. ^*P < 0.05 vs. si-NC group, ^#P < 0.05 vs. si-miR group. (F) The apoptosis-associated protein Bcl2, Bax, and Cytochrome-c (Cyt-c) levels were detected in CRC cells. n = 5. ^*P < 0.05, ^**P < 0.01 vs. si-NC group, ^#P < 0.05 vs. si-miR group. (G) The LC3 level was detected by immunofluorescence. n = 5. ^*P < 0.05 vs. si-NC group, ^#P < 0.05 vs. si-miR group. (H) The protein level of LC3 in CRC cells. n = 5. ^*P < 0.05 vs. si-NC group, ^#P < 0.05 vs. si-miR group.