Research Paper Volume 14, Issue 1 pp 497—508

Figure 2. circCUL2 inhibits tumor progression via targeting miR-208a-3p. (A) CCK-8 assay was performed to detect the cell viability in CRC cells. n = 6. ^*P < 0.05. (B) Cell cycle was determined in CRC cells by flow cytometry. n = 5. ^*P < 0.05 vs. NC group, ^#P < 0.05 vs. circCUL2 group. n.s. indicated no significance. (C) The clone formation assay was used to assess the cell proliferation. (D) The EdU assay results in CRC cells. n = 5. ^*P <0.05 vs. NC group, ^#P < 0.05 vs. circCUL2 group. (E) The flow cytometry was performed to detect the apoptosis level in CRC cells. n = 5. ^*P < 0.05 vs. NC group, ^#P < 0.05 vs. circCUL2 group. (F) The apoptosis-associated protein Bcl2, Bax, and Cytochrome-c (Cyt-c) levels were detected in CRC cells. n = 5. ^*P < 0.05 vs. NC group, ^#P < 0.05 vs. circCUL2 group. (G) Mitochondrial-Bcl2 and Bax levels was detected in CRC cells. n = 4. ^*P < 0.05 vs. NC group, ^#P < 0.05 vs. circCUL2 group.