Research Paper Volume 14, Issue 1 pp 443—461
PEGylation and antioxidant effects of a human glutathione peroxidase 1 mutant
Figure 6. Determination of cell viability in vitro. Cytotoxic effects of cotreatment with GPx1M, SS-mPEG-GPx1M (A), and ADR (B) on H9c2 cells. (C) Cell viabilities of H9c2 cells that were preincubated with different concentrations of GPx1M and SS-mPEG-GPx1M (0.01–0.4 U/mL) for 1 h, and then treated with ADR (2.5 μM) for 24 h. (D) Cell viabilities of H9c2 cells that were treated with ADR (2.5 μM) for 12 h, and then co-incubated with different concentrations of GPx1M and SS-mPEG-GPx1M (0.01–0.4 U/mL) for another 12 h.