Research Paper Volume 14, Issue 1 pp 410—429
Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: in vitro and in vivo studies
Figure 4. Effect of FOXO1 on endothelial cells treated with GCs. (A) predicted target gene of miR-135b through miRwalk, TargetScan, and miRDB; GO and KEGG enrichment analysis of potential target genes via ClueGO; bioinformatics analysis revealed FOXO1 as a potential target gene of miR-135b. (B) dual luciferase reporter assay verified the targeting relationship between miR-135b and FOXO1; After overexpression of FOXO1, ECs were subjected to ESWT with 0.05 mJ/mm2, 1000 shots followed by DEX with 180 μM. (C) cell viability examined by CCK-8 analysis in BMECs; (D) cell proliferation confirmed by EdU assay in BMECs; After overexpression of FOXO1, ECs were subjected to ESWT with 0.03 mJ/mm2, 1000 shots followed by DEX with 180 μM. (E) the apoptosis rate assessed through Annexin V-FITC/PI in BMECs; After overexpression of FOXO1, ECs were subjected to ESWT with 0.05 mJ/mm2, 1000 shots followed by DEX with 180 μM. (F) migration ability evaluated by wound healing assay in BMECs; (G) migration ability evaluated by Transwell assay in BMECs; (H) angiogenesis ability evaluated by tube formation assay in BMECs. n=3 **P < .01, ***P < .001.