Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: <i>in vitro</i> and <i>in vivo</i> studies

Xinjie Wu; Yanlei Wang; Xiaoyu Fan; Xin Xu; Wei Sun

doi:doi:10.18632/aging.203816

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Research Paper Volume 14, Issue 1 pp 410—429

Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: in vitro and in vivo studies

Figure 2. Effect of ESWT on endothelial cells treated with GCs. (A–C) cell viability was examined by CCK-8 analysis in HUVECs; ECs were subjected to ESWT with 0.05 mJ/mm², 1000 shots followed by DEX with 180 μM. (D) cell viability examined by CCK-8 analysis in BMECs; (E) cell proliferation confirmed by EdU assay in BMECs; ECs were subjected to ESWT with 0.03 mJ/mm², 1000 shots followed by DEX with 180 μM. (F) apoptosis rate of assessed through Annexin V-FITC/PI in BMECs; ECs were subjected to ESWT with 0.05 mJ/mm², 1000 shots followed by DEX with 180 μM. (G) migration ability evaluated by wound healing assay in BMECs; (H) migration ability evaluated by Transwell assay in BMECs; (I) angiogenesis ability evaluated by tube formation assay in BMECs. n=3 ^**P < .01, ^***P < .001.