Research Paper Volume 14, Issue 1 pp 368—388

LINC02362 attenuates hepatocellular carcinoma progression through the miR-516b-5p/SOSC2 axis

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Figure 2. LINC02362 suppresses HCC cell survival. (A) RT-qPCR quantification (n=3) of LINC02362 expression in Hep3B or PLC/PRF/5 cells with LINC02362 ectopic expression. (B) MTT assay (n=3) for measuring the proliferative abilities of HCC cells with LINC02362 overexpression. (C, D) EdU labeling to detect the percentage of dividing cells in LINC02362-overexpressing HCC cells and the corresponding quantification (D; n=3). (E, F) Measurement (E) and quantification (F; n=3) of cell cycle of HCC cells by flow cytometry. (G, H) Detection (G) and quantification (H; n=3) of apoptotic cells in HCC cells overexpressing LINC02362. *0.01 < P < 0.05, **0.001 < P < 0.01, ***0.0001 < P < 0.001, ****P < 0.0001.