Research Paper Volume 13, Issue 24 pp 26137—26147

Figure 3. miR-545 decreased lipid oxidation and iron accumulation in HT-29 and HCT-116 cells. An erastin and RSL3-induced increase in MDA and ROS levels was significantly reduced in HT-29 and HCT-116 cells transfected with OV-pre-miR-545 (A), while inhibition of miR-545 further increased MDA and ROS levels (B), in both HT-29 and HCT-116 cells. ROS levels were reduced in HT-29 and HCT-116 cells transfected with OV-pre-miR-545 (C), but inhibition of miR-545 further increased ROS levels (D), in both HT-29 and HCT-116 cells. (E) Levels of ferrous iron (Fe2+) were decreased in HT-29 and HCT-116 cells transfected with OV-pre-miR-545 following treatment with erastin and RSL3. (F) Anti-miR-545 further increased Fe2+ levels in erastin and RSL3-treated HT-29 and HCT-116 cells. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. as indicated.