Research Paper Volume 13, Issue 24 pp 26137—26147
miR-545 promotes colorectal cancer by inhibiting transferring in the non-normal ferroptosis signaling
Figure 2. miR-545 increased erastin and RSL3-induced ferroptosis in HT-29 and HCT-116 cells. (A) Erastin and RSL3 significantly decreased cell survival rate in a dose-dependent manner. (B) RT-PCR showed that transfection with OV-pre-miR-545 significantly increased miR-545 levels in HT-29 and HCT-116 cells. (C) Pretreatment with miR-545 significantly increased HT-29 and HCT-116 cell survival rates. (D) Transfection with anti-miR-545 significantly reduced miR-545 levels in HT-29 and HCT-116 cells. (E) CCK-8 assay demonstrated that miR-545 knockdown further reduced the erastin and RSL3-induced decrease in cell survival rate. *p < 0.05, **p < 0.01, ***p < 0.001 vs. as indicated.