Research Paper Volume 13, Issue 24 pp 26095—26117
Knockdown hsa_circ_0063526 inhibits endometriosis progression via regulating the miR-141-5p / EMT axis and downregulating estrogen receptors
Figure 6. EdU assay was used to investigate the effect of inhibition of hsa_circ_0063526 or/and miR-141-5p on the proliferation of End1/E6E7 cells. (A) si-hsa_circ_0063526 group; (B) si-hsa_circ_0063526 +inhibitor miR-141-5p group; (C) miR-141-5p Inhibitor group; (D) NC-RNA group; (E) Blank control group; (F) the number of red fluorescent cells. After transfection with hsa_circ_0063526 siRNA in End1/E6E7 cells, the number of cells in the control group was lower, and the proliferation ability of cells in endometriosis was decreased. After co-transfection with si-hsa_circ_0063526 + inhibitor miR-141-5p, the proliferation ability of End1/E6E7 cells was rescued. Replicated 3 times with 3 separate samples (* P<0.05, ** P<0.01) (bar:25μm).