Figure 2. Reduction in neuronal loss in the cortical and hippocampal neurons of P8 mice after rTMS treatment. (A) The changes in the morphology of cortical and hippocampal neurons in mice were evaluated by HE staining (scale bar, 20μm). (B) TUNEL staining was performed for the mouse cortical and hippocampal regions (scale bar, 20μm, magnification, 10x eye piece x 40x objective). (C) Histogram analysis of TUNEL-positive cells. The data is demonstrated as the mean ± standard deviation (n =5). *P < 0.05 vs. Con group; # P < 0.05 vs. P8 group. TUNEL, the terminal deoxynucleotidyl transferase nick-labelling; HE, haematoxylin and eosin.