Research Paper Volume 14, Issue 1 pp 297—315

Figure 2. Upregulation of TIMP-2 in 5-Fu resistant CRC cells in vitro. (A, B) Relative cell viabilities of DLD-1 5-FuS cells and DLD-1 5-FuR cells, HCT116 5-FuS cells and HCT116 5-FuR cells under increasing concentrations of 5-Fu for 3 days. (C, D) Relative cell viabilities of DLD-1 5-FuS cells and HCT116 5-FuS cells in increasing concentrations of 5-Fu for 3 days after culture in conditioned medium of DLD-1 5-FuR cells or HCT116 5-FuR cells for 2 days. (E) mRNA expression levels of TIMP-2 in paired DLD-1 5-FuS cells and DLD-1 5-FuR cells, HCT116 5-FuS cells and HCT116 5-FuR cells. (F) Differences in TIMP-2 protein expression levels in paired DLD-1 5-FuS cells and DLD-1 5-FuR cells, HCT116 5-FuS cells and HCT116 5-FuR cells. Data from triplicate wells in 3 independent experiments. (A, B) ^***p < 0.001 by 2 way ANOVA test. (C–F) ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 by Student’s t-test.